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Puji Lestari 1*), I. Roostika 1), Kristianto Nugroho 1), Edison HS 2), Habib Rijzaani 1) and Mastur 1)
1) Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, West Java, Indonesia
2) Indonesian Tropical Fruit Research Institute, Solok, West Sumatera, Indonesia

Molecular marker is robust to precisely monitor the genetic stability of in vitro-banana plants. This study examined the genetic stability of 8 month- old banana plants of Soka variety derived from floral axis organogenesis using newly developed SSR markers. The results showed that the same qualitative and similar quantitative morphological characters of pseudostem, leaf and fruit were identified between mother plants and culture plants from floral axis regeneration. Both plants types were quite similar in number of tillers, brix percentage, fruit peel/mesocarp thickness and fruit length. Eleven out of 211 good quality of SSR loci showing high homology with important genes were selected for suitable PCR primers and produced unambiguous bands.The number of total bands was 323 for total SSR primers, in range of 20-60 per primer for total individual plants. Most culture plants showed identical with their mother plants, with very minor variation as reflected by genetic similarity coefficient range of 0.9-1.0. A high similar pattern on SSR to support morphological characters of mother plants and culture plants indicated a successful micropropagation using floral axis to encounter off-type clones.The floral axis organogenesis in this study is able to provide sufficient genetic materials of Soka for varietal registration and other applications

AGRIVITA Journal of Agricultural Science. 2019. 41(2): 302–315


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Affandi 1), Dhasa Ustistio 2), Panca Jarot Santoso 1), Ellina Mansyah 1) and Dewi Sart)iami
1) Indonesian Tropical Fruits Research Institute, Solak, West Sumatra, Indonesia
2) Faculty of Agnculture and Animal Husbandry, Islamic University of Sultan Syarif Kasun. Pekanbaru, Rrau, Indonesia
3) Department of Plant Protection. Bogor Agncultural University, Bogar, West Java, Indonesia


Durian contnbutes  a high economic value 1n agribusiness of horticultural product.  However,  recently the attack  of scale  insect has devastated most of pre-prccuctton trees  in  Indonesia.   A research  with  objective to know  the  basic information  about the  correct speaes,  orstnbution and  population  abundance  was  done  at Anpan  Research  Station  of Indonesian   Tropical   Fruits   Research  Institute   from  July  to  August 2018. A proper ldennficaucn  and  observation  method  were  used  to know  the  species identity,  distribution  and  abundance  of the  insect. Biotic  and  abronc  factors  in  each  done   such  as  nutnents content, tree   morphological    charactensuc,   hght   intensity,   temperature   and relative  humidity   under  canopy  were  analysed.  The  result  showed that Aulacasp1s  v1lts  Green  was  a scale  insect  identified  associating with  durian  leaves.   Clone Bintana and Sikapal were the most and the less  preferred  wit the intensity  of damage  was  88.60  % and  38.30%, respectively.   Nutnent content especially nitrogen and water as well as  canopy  morphology  were  simultaneously the  most  contributed  m the preference of a certain clone.   The pest was preferred  to dump  m the East cardinal direction and no significant drfference between  upper and  lower  part of the tree  includmg  within  and outside  of the canopy.

 AGRIVITAJoumal  of Agricultural  Science.  2019   42(2)·  1-2


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