Publikasi
Puji Lestari 1*), I. Roostika 1), Kristianto Nugroho 1), Edison HS 2), Habib Rijzaani 1) and Mastur 1)
1) Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, West Java, Indonesia
2) Indonesian Tropical Fruit Research Institute, Solok, West Sumatera, Indonesia
ABSTRACT
Molecular marker is robust to precisely monitor the genetic stability of in vitro-banana plants. This study examined the genetic stability of 8 month- old banana plants of Soka variety derived from floral axis organogenesis using newly developed SSR markers. The results showed that the same qualitative and similar quantitative morphological characters of pseudostem, leaf and fruit were identified between mother plants and culture plants from floral axis regeneration. Both plants types were quite similar in number of tillers, brix percentage, fruit peel/mesocarp thickness and fruit length. Eleven out of 211 good quality of SSR loci showing high homology with important genes were selected for suitable PCR primers and produced unambiguous bands.The number of total bands was 323 for total SSR primers, in range of 20-60 per primer for total individual plants. Most culture plants showed identical with their mother plants, with very minor variation as reflected by genetic similarity coefficient range of 0.9-1.0. A high similar pattern on SSR to support morphological characters of mother plants and culture plants indicated a successful micropropagation using floral axis to encounter off-type clones.The floral axis organogenesis in this study is able to provide sufficient genetic materials of Soka for varietal registration and other applications
AGRIVITA Journal of Agricultural Science. 2019. 41(2): 302–315
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Affandi 1), Dhasa Ustistio 2), Panca Jarot Santoso 1), Ellina Mansyah 1) and Dewi Sart)iami
1) Indonesian Tropical Fruits Research Institute, Solak, West Sumatra, Indonesia
2) Faculty of Agnculture and Animal Husbandry, Islamic University of Sultan Syarif Kasun. Pekanbaru, Rrau, Indonesia
3) Department of Plant Protection. Bogor Agncultural University, Bogar, West Java, Indonesia
ABSTRACT
Durian contnbutes a high economic value 1n agribusiness of horticultural product. However, recently the attack of scale insect has devastated most of pre-prccuctton trees in Indonesia. A research with objective to know the basic information about the correct speaes, orstnbution and population abundance was done at Anpan Research Station of Indonesian Tropical Fruits Research Institute from July to August 2018. A proper ldennficaucn and observation method were used to know the species identity, distribution and abundance of the insect. Biotic and abronc factors in each done such as nutnents content, tree morphological charactensuc, hght intensity, temperature and relative humidity under canopy were analysed. The result showed that Aulacasp1s v1lts Green was a scale insect identified associating with durian leaves. Clone Bintana and Sikapal were the most and the less preferred wit the intensity of damage was 88.60 % and 38.30%, respectively. Nutnent content especially nitrogen and water as well as canopy morphology were simultaneously the most contributed m the preference of a certain clone. The pest was preferred to dump m the East cardinal direction and no significant drfference between upper and lower part of the tree includmg within and outside of the canopy.
AGRIVITAJoumal of Agricultural Science. 2019 42(2)· 1-2
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