Ditulis oleh Administrator Dilihat: 47

P. MURNI1, SYAMSUARDI2*, NURAINAS2, E. MANSYAH³ and M. HUDA²
1Department of Biology, Faculty of Education, Universitas Jambi, Indonesia
2Departement of Biology, Faculty of Mathematics & Natural Sciences, Universitas Andalas, Indonesia
3Indonesian Tropical Fruit Research Institute, Solok, West Sumatra, Indonesia
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SUMMARY
Duku ‘Kumpeh’ is one of the superior and potential cultivar of Duku (Lansium parasiticum) that originate from a specific area in the Jambi and it becomes one of the essential sources for income of Jambi people. For sustainable utilization of this essential local germplasm of tropical fruit need the understanding of the phenomenon of polyembryony and genetic information of the plant. The study of genetic variation of multiple seedlings of duku from Kumpeh was conducted. In this study, 34 seedlings were used (14 seedlings from Kumpeh and 20 seedlings from Dusun Tuo) from two mother trees in order to collect the samples of DNA. Extracted DNA samples used a modification of CTAB method then amplified using four primers of Inter-Simple Sequence Repeat (ISSR) namely (AAC)6, (AAG)6, (AAT)10, (AG)10 and two from RAPD markers (OPA 13 and OPA 18) to asses genetic variation. Base on observed and analysis of genetic variation of samples showed that multiple seedlings of seven seeds (14 seedling individuals) from Kumpeh and ten seeds (14 seedling individuals from Dusun Tuo) have the uniform of DNA band profile and the same as those of mother trees. The existing identical of all seedlings suggested that polyembryony of duku seeds derived from the non-zygotic embryo. Thus, we concluded that the reproduction system of duku (Lansium parasiticum (Osbeck) K.C. Sahni and Bennet.) occurred asexual with apomictic reproduction.

Key words: Lansium parasiticum, genetic uniformity, polyembryony, non-zygotic embryo

SABRAO Journal
of Breeding and Genetics
51 (2) 151-160, 2019

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