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Pinta Murni1, Syamsuardi2*, Nurainas2, Ellina Mansyah3 and Chairul2
1) Doctor Student of Biology Study Program, Faculty of Math. & Nat. Sci. Andalas University & Department Biology,
2) Fac. Faculty of Math. Nath. Jambi University; 2Department of Biology, Faculty of Math. & Nat. Sci. Andalas University;
3 )Indonesian Tropical Fruit Research Institute, Solok, West Sumatra

ABSTRACT
Duku ‘Kumpeh’ is one of the potential local type cultivars of Duku Lansium parasiticum (Osbeck) K.C. Sahni & Bennet.), from specific area in the Jambi. For sustainable utilization of this local germplasm of tropical fruit, understanding of reproduction and genetic information of the plant is needed. The reproductive system of a plant species is a factor that directly determine the potential and success of conservation germplasm for the future. Until now, the breeding system about this plant still unclear although there were different opinions whether duku produce asexually (apomictic), sexual or both. Based on this fact, it is necessary to clarify this problem. A total of 60 seeds from three parental trees (20 seeds from Kumpeh and 22 seeds from Dusun Tuo and 18 seeds from Muaro Panco) were germinated in the greenhouse for obtaining DNA samples. DNA samples were extracted using CTAB method and amplified using Inter Simple Sequences Repeat (ISSR) primers. Four selected primers: (CCT)8 (HQ200181), (AAT)10 (HQ200186), (AAG)6 (HQ200182) and (AG)10 (DQ453906) were used to amplify all DNA samples and estimate the magnitude of genetic variability and out-crossing rate. Based on analysis of genetic variability and assessment of level out-crossing rate of three parental and sixty progenies from three populations proved that local cultivar ‘Kumpeh’ were facultative apomictic breeding system. The existing identical of all progenies in Kumpeh population with their parental were very important for sustainably utilization of this local type duku ‘Kumpeh’. The detection genetic variability (even with low level) at Dusun Tuo and Muaro Panco population were very useful and strategic for genetic conservation and genetic improvement of these germplasm.

Keywords : Apomictic, duku ’kumpeh’, genetic variability, ISSR, Lansium parasiticum, out-crossing rate.

Scholars Research Library
Der Pharmacia Lettre, 2016, 8 (18):185-191
(http://scholarsresearchlibrary.com/archive.html)

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P. MURNI1, SYAMSUARDI2*, NURAINAS2, E. MANSYAH³ and M. HUDA²
1Department of Biology, Faculty of Education, Universitas Jambi, Indonesia
2Departement of Biology, Faculty of Mathematics & Natural Sciences, Universitas Andalas, Indonesia
3Indonesian Tropical Fruit Research Institute, Solok, West Sumatra, Indonesia
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SUMMARY
Duku ‘Kumpeh’ is one of the superior and potential cultivar of Duku (Lansium parasiticum) that originate from a specific area in the Jambi and it becomes one of the essential sources for income of Jambi people. For sustainable utilization of this essential local germplasm of tropical fruit need the understanding of the phenomenon of polyembryony and genetic information of the plant. The study of genetic variation of multiple seedlings of duku from Kumpeh was conducted. In this study, 34 seedlings were used (14 seedlings from Kumpeh and 20 seedlings from Dusun Tuo) from two mother trees in order to collect the samples of DNA. Extracted DNA samples used a modification of CTAB method then amplified using four primers of Inter-Simple Sequence Repeat (ISSR) namely (AAC)6, (AAG)6, (AAT)10, (AG)10 and two from RAPD markers (OPA 13 and OPA 18) to asses genetic variation. Base on observed and analysis of genetic variation of samples showed that multiple seedlings of seven seeds (14 seedling individuals) from Kumpeh and ten seeds (14 seedling individuals from Dusun Tuo) have the uniform of DNA band profile and the same as those of mother trees. The existing identical of all seedlings suggested that polyembryony of duku seeds derived from the non-zygotic embryo. Thus, we concluded that the reproduction system of duku (Lansium parasiticum (Osbeck) K.C. Sahni and Bennet.) occurred asexual with apomictic reproduction.

Key words: Lansium parasiticum, genetic uniformity, polyembryony, non-zygotic embryo

SABRAO Journal
of Breeding and Genetics
51 (2) 151-160, 2019

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